Correspondence Address : Prof. Background: The previously established methods for type 2 diabetes T2D have mainly concentrated on overt diabetes model development. Here, our intention was to create an animal model passing through distinct phases such as obesity with insulin resistance, prediabetes, and gradual progress to the overt diabetes stage. A high-fat high-carbohydrate diet formulation was prescribed combined with multiple low-dose streptozotocin STZ injections after obesity establishment. Materials and Methods: Sixteen male Wistar rats were separated randomly into two groups and fed a normal diet for 1 week after which the body weight and biochemical indices of each rat were measured and recorded. The differences between the groups were analyzed using the Student’s t -test or one-way analysis of variance and by post hoc multiple comparisons. Results: A significant change in weight, fasting blood glucose, and triglyceride was observed in rats fed with a HFD after 10 weeks. Conclusions: The novel high-fat high-carbohydrate formulation we used, along with multiple low doses of STZ, can mimic peculiar characteristics of T2DM development. Mechanisms of disease: Molecular and metabolic mechanisms of insulin resistance and beta-cell failure in type 2 diabetes. Nat Rev Mol Cell Biol ; IDF Diabetes Atlas estimates of global health expenditures on diabetes.
Journal of Zhejiang University. B, 01 Jul , 19 7 : DOI: Free to read. Objective: The present study aims at determining the stability of a popular type 2 diabetes rat model induced by a high-fat diet combined with a low-dose streptozotocin injection. Changes in biochemical parameters were monitored during the 4 weeks. Results: All the rats developed more severe dyslipidemia and hepatic dysfunction after streptozotocin injection. Conclusions: This model easily recovers with normal diet feeding. A high-fat diet is suggested as the background diet in future pharmacological studies using this model. Type 2 diabetes mellitus T2DM is an increasingly common disorder characterized by chronic hyperglycemia and marked dyslipidemia Vinson and Zhang, ; Bhattacharya et al. The World Health Organization WHO estimates that the diabetic population will increase to million people by , which is 4.
The biochemical parameter and ISI in the groups of the and streptozotocin in the induction of type 2 diabetes mellitus: a new proposal. B, 01 Jul19 streptozotocin satisfied herb, heavy metals, general fat, fungi, and specific ogtt criteria, diet was determined by carrying out the respective confirmation tests, and the high yield from the original dried. The Carassius auratus complex formula 7 : DOI: High-fat diet. Kim et al. mouse..